+**Af4** (Asymmetric Flow Field-Flow Fractionation) is a gentle separation technique used to analyze complex mixtures of [Macromolecules](/wiki/Macromolecules) and [Nanoparticles](/wiki/Nanoparticles). It separates components based on their diffusion coefficient, offering high resolution without harsh shear forces.
+## See also
+- [Separation Science](/wiki/Separation_Science)
+- [Biophysics](/wiki/Biophysics)
+- [Chromatography](/wiki/Chromatography)
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